Biological conversion assay using Clostridium phytofermentans to estimate plant feedstock quality

Biological conversion assay using Clostridium phytofermentans to estimate plant feedstock quality

BACKGROUND There is currently considerable interest in developing renewable sources of energy. One strategy is the biological conversion of plant biomass to liquid transportation fuel. Several technical hurdles impinge upon the economic feasibility of this strategy, including the development of energy crops amenable to facile deconstruction. Reliable assays to characterize feedstock quality are...

Genome and Transcriptome of Clostridium phytofermentans, Catalyst for the Direct Conversion of Plant Feedstocks to Fuels

Clostridium phytofermentans was isolated from forest soil and is distinguished by its capacity to directly ferment plant cell wall polysaccharides into ethanol as the primary product, suggesting that it possesses unusual catabolic pathways. The objective of the present study was to understand the molecular mechanisms of biomass conversion to ethanol in a single organism, Clostridium phytofermen...

ethanol - tolerant strain of Clostridium phytofermentans

Characterization of Three -Galactoside Phosphorylases from Clostridium phytofermentans

We characterized three D-galactosyl133-N-acetyl-Dhexosamine phosphorylase (EC 2.4.1.211) homologs from Clostridium phytofermentans (Cphy0577, Cphy1920, and Cphy3030 proteins). Cphy0577 and Cphy3030 proteins exhibited similar activity on galacto-N-biose (GNB; D-Gal133-D-GalNAc) and lacto-N-biose I (LNB; D-Gal133-DGlcNAc), thus indicating that they are D-galactosyl133-Nacetyl-D-hexosamine phospho...

Clostridium phytofermentans sp. nov., a cellulolytic mesophile from forest soil.

An obligately anaerobic, mesophilic, cellulolytic bacterium, strain ISDgT, was isolated from forest soil. Cells of this isolate stained Gram-negative, despite possessing a Gram-positive cell-wall ultrastructure, and were motile, straight rods that formed spherical terminal spores that swelled the sporangium. Cellulose, pectin, polygalacturonic acid, starch, xylan, arabinose, cellobiose, fructos...